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ad-gfp  (Vector Biolabs)


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    Structured Review

    Vector Biolabs ad-gfp
    Ad Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 369 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ad-gfp/Ad-GFP/custom%401060%4042532545
    Average 96 stars, based on 369 article reviews
    ad-gfp - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Plasmid Preparation:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Recombinant:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Expressing:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Incubation:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Infection:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    RNA Extraction:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Extraction:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Virus:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Cell Culture:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    shRNA:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Transfection:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Quantitative RT-PCR:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Isolation:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Mouse Assay:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Negative Control:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ

    Control:

    Article Title: Modeling maternal immune activation in 3D ex vivo human fetal brain cerebroids reveals IL-17A-driven disruption of cortical development.
    Article Snippet: 40) according to the manufacturer’s instructions. Genetic labeling of radial glia cells using adenovirus The recombinant human Adenovirus Type 5 (dE1/E3)-expressing enhanced green fluorescent protein (Vector Biolabs, cat. no. 1060) or red fluorescent protein (Vector Biolabs, cat. no. 1660) under CMV promoter was used to selectively label apical radial glia cells in the cerebroids. We added ~15–20 μ



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    Image Search Results


    Primary cilia are required for GLP-1-potentiated insulin secretion. ( A ) Dynamic insulin secretion from perifused mouse islets. Wild-type (WT, black) and β-cell-specific cilia knockout (βCKO, red) islets were sequentially exposed to 2 mM glucose (2G), 16 mM glucose (16G), 20 nM liraglutide (Lira), and 30 mM KCl as indicated. n = 6 replicates of 50 islets per genotype from 5 mice. ∗p < 0.05, two-way ANOVA with Sidak's multiple comparisons test. ( B ) Quantitative analysis of secretion traces: area under the curve (AUC) during initial glucose stimulation (minutes 10–30), liraglutide stimulation (minutes 30–50), KCl depolarization (minutes 60–70), and total AUC of the entire perifusion (minutes 0–70). βCKO islets secreted significantly less insulin in response to glucose and liraglutide but showed no defect in KCl-induced secretion. Data are mean ± SEM. ∗∗p < 0.01; ns, not significant, unpaired student's t-test. ( C-D ) IFT88 knockdown impairs GLP-1-augmented secretion in human islets. Static glucose-stimulated insulin secretion (GSIS) assays from islets of three male ( C ) and three female ( D ) donors. Islets transduced with control (Ctl, white) or IFT88 -targeting shRNA (IFT88 KD, red) were incubated at 1 mM glucose (1G), 11 mM glucose (11G), and 11G + 100 nM liraglutide (Lira). Donor ages are indicated. IFT88 KD significantly reduced liraglutide-potentiated insulin secretion. Data are mean ± SEM of triplicate samples per donor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant; one-way ANOVA with Tukey's multiple comparisons test.

    Journal: Molecular Metabolism

    Article Title: Primary cilia regulate GLP-1 signaling in pancreatic β cells

    doi: 10.1016/j.molmet.2026.102357

    Figure Lengend Snippet: Primary cilia are required for GLP-1-potentiated insulin secretion. ( A ) Dynamic insulin secretion from perifused mouse islets. Wild-type (WT, black) and β-cell-specific cilia knockout (βCKO, red) islets were sequentially exposed to 2 mM glucose (2G), 16 mM glucose (16G), 20 nM liraglutide (Lira), and 30 mM KCl as indicated. n = 6 replicates of 50 islets per genotype from 5 mice. ∗p < 0.05, two-way ANOVA with Sidak's multiple comparisons test. ( B ) Quantitative analysis of secretion traces: area under the curve (AUC) during initial glucose stimulation (minutes 10–30), liraglutide stimulation (minutes 30–50), KCl depolarization (minutes 60–70), and total AUC of the entire perifusion (minutes 0–70). βCKO islets secreted significantly less insulin in response to glucose and liraglutide but showed no defect in KCl-induced secretion. Data are mean ± SEM. ∗∗p < 0.01; ns, not significant, unpaired student's t-test. ( C-D ) IFT88 knockdown impairs GLP-1-augmented secretion in human islets. Static glucose-stimulated insulin secretion (GSIS) assays from islets of three male ( C ) and three female ( D ) donors. Islets transduced with control (Ctl, white) or IFT88 -targeting shRNA (IFT88 KD, red) were incubated at 1 mM glucose (1G), 11 mM glucose (11G), and 11G + 100 nM liraglutide (Lira). Donor ages are indicated. IFT88 KD significantly reduced liraglutide-potentiated insulin secretion. Data are mean ± SEM of triplicate samples per donor. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001; ns, not significant; one-way ANOVA with Tukey's multiple comparisons test.

    Article Snippet: IFT88 knockdown in human islets: Healthy non-diabetic human islets were transduced with adenoviral vectors encoding either GFP-tagged shRNA targeting human IFT88 (Ad-GFP-h-IFT88-shRNA) or scrambled control (Ad-GFP-U6-scrmb-shRNA, #1122N; Vector Biolabs).

    Techniques: Knock-Out, Knockdown, Transduction, Control, shRNA, Incubation